Plant Biotechnology

  • Author(s): S Umesha
  • Size: 160 mm × 240 mm
  • Pages: 438
  • Binding: Paperback
  • Language: English
  • ISBN: 9788179935033
  • Cover Price:

    Rs.795.00 / US $48.00

  • Special Price: Rs. 715.00 / US $27.00

Book Details

Plant biotechnology is a precise science which allows for the transfer of only one or a few desirable genes. It is through this precise science that plant breeders are able to develop crops with specific beneficial and desirable traits. Thus, plant biotechnology an important aspect of agriculture.

This book comprehensively covers almost every aspect of plant biotechnology—from tissue culture and its applications to plant transformation technology and molecular marker aided breeding. It also addresses the most recent advances in this much needed science.

Key Features

  • Comprehensive coverage of monitoring and evaluation of afforestation activities.
  • Focus on various aspects of monitoring and evaluation of afforestation.
  • Brief provided on the National Afforestation Project, including its intended monitoring and evaluation process and suggested modifications.

Target Audience

    Postgraduates, researchers, academicians, and professionals of plant biotechnology, plant biology, biochemistry, molecular biology, pharmacology, agronomy, genetics; ethnobotany, ecology; nutritionists; pharmacists; plant breeders; ecologists, and conservationists

Table of Contents

    1. Tissue Culture
    1.1 Introduction to Cell and Tissue Culture
    1.2 Tissue Culture Media (Composition and Preparation)
    1.3 Stock Solutions
    1.4 Sterilization and Agents of Sterilization used in Tissue Culture Laboratories
    1.5 Initiation and Maintenance of Callus and Cell Suspension Culture
    1.6 Nutritional Requirement for in-vitro Culture
    1.7 Concept of Cellular Totipotency
    1.8 Single Cell Clones and Organogenesis
    1.9 Organogenesis
    1.10 Artificial Seeds
    1.11 Micropropagation
    1.12 Somatic Embryogenesis
    1.13 Transfer and Establishment of Whole Plants in Soil
    1.14 Shoot Tip Culture
    1.15 Rapid Clonal Propagation and Production of Virus-free Plants
    1.16 Embryo Culture and Embryo Rescue
    1.17 Protoplast Culture and Fusion
    1.18 In vitro Mutation Methods
    1.19 Selection of Hybrid Cells and Regeneration of Hybrid Plants
    1.20 Symmetric and Asymmetric Hybrids
    1.21 Cybrid Production and their Application in Crop Improvement
    1.22 Cryobiology of Plant Cell Culture and Establishment of Gene Bank
    1.23 Production of Virus-free Plants using Meristem Culture
    1.24 Anther, Pollen, and Ovary Culture for Production of Haploid Plants and Homozygous Lines

    2. Tissue-culture Applications
    2.1 Regeneration
    2.2 Production of Haploids
    2.3 Germplasm Conservation
    2.4 Production of Secondary Metabolites from Plant Cell Culture
    2.5 Industrial Production of Useful Biochemicals by Higher-plant Cell Cultures
    2.6 Industrial Production of Food Additives and Fragrances
    2.7 Bioreactor Systems
    2.8 Greenhouse Technology

    3. Plant Transformation Technology
    3.1 Introduction
    3.2 Hairy Root
    3.3 Structure and Functions of Ti and Ri Plasmids and their Application
    3.4 Agrobacterium-Mediated Gene Transfer, Agrobacterium-Based Vectors, and their Application
    3.5 Binary Vectors
    3.6 Uses of 35S Promoter
    3.7 Reporter Genes
    3.8 Matrix Attachment Region
    3.9 Biolistic Gene Transfer
    3.10 Transgene Stability and Gene Silencing

    4. Plant Genetic Engineering for Productivity and Performance
    4.1 Resistance to Herbicides
    4.2 Insect Resistance
    4.3 Abiotic Stress—Salt

    5. Molecular Marker-aided Breeding
    5.1 Molecular Markers
    5.2 DNA Extraction—Beginning of Molecular Markers Analysis
    5.3 Types of Molecular Markers
    5.4 Types of Markers
    5.5 Restriction Fragment Length Polymorphism
    5.6 Linkage Analysis
    5.7 RAPD Markers
    5.8 Microsatellites
    5.9 Sequence-characterized Amplified Region
    5.10 Single Nucleotide Polymorphism
    5.11 Amplified Fragment Length Polymorphism
    5.12 Quantitative Trait Loci
    5.13 Mapping Functions

    6. Application of Plant Biotechnology
    6.1 Introduction
    6.2 Industrial Enzymes
    6.3 Genetic Engineering
    6.4 Edible Vaccines
    6.5 Plantibodies
    6.6 Phenylpropanoid Pathway
    6.7 Shikimate Pathway
    6.8 Transgenics

    7. Biotechnological Applications
    7.1 Mass Multiplication of Economically Important Plants
    7.2 Biopesticides (Insecticides)
    7.3 Biofertilizers
    7.4 Vermiculture
    7.5 Phytoremediation
    7.6 Nutraceuticals
    7.7 Cosmeceuticals
    7.8 Biofuels
    7.9 Single-Cell Protein

    8. Important Issues in Plant Biotechnology
    8.1 Introduction
    8.2 Commercial Status and Public Acceptance
    8.3 Biosafety Guidelines for Research Involving the Benefits and Risks of Genetically Modified Organisms
    8.4 Socio-Economic Impacts and Ecological Consideration of Genetically Modified Organisms
    8.5 Private Sector, Trade Regulations, and Intellectual Property Rights
    8.6 Genetic use Restriction Technology
    8.7 Biosafety
    8.8 Weaknesses in Testing and Regulations
    8.9 Public Participation
    8.10 Gene Flow, IPR, and IPP


    About the Author


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