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Bioanalytical Techniques

  • Author(s): Abhilasha Shourie and Shilpa S Chapadgaonkar
  • Size: 180 mm x 240 mm
  • Pages: 411
  • Binding: Paperback
  • Language: English
  • ISBN: 9788179935293
  • Cover Price:

    Rs.395.00 / US $28.00

  • Special Price: Rs. 355.00 / US $25.00

Book Details

Bioanalytical Techniques form an integral part of applied biology and biomedical sciences. The book provides understanding of the concept and working principles of various bioanalytical  techniques used in biomedical sciences, environmental studies, life sciences, pharmaceutical analysis, molecular biology, and biotechnological research, as well as the various instruments used in these processes. Divided into 12 chapters, the book provides a comprehensive account of microscopy, centrifugation, chromatography, electrophoresis, spectroscopy. It also focuses on two main topics: radioisotope and immunodiagnostic techniques. Techniques in molecular biology and recombinant DNA technology have also been described in detail.

Key Features

    • Explains analytical instrumentation in a concise manner
    • Provides state-of-the-art sophisticated techniques that would be beneficial to researchers in various fields for experimentation
    • Encourages reader to analytical thinking and practical application of the technique

Target Audience

    Graduate and Postgraduate students, Researchers, Academicians, and Professionals in the fields of Environmental Studies, Chemistry, Analytical Chemistry, Biomedical Science, Pharmacy, Bioanalysis, Serology, Pharmaceutical Chemistry, Pharmaceutical Analysis, Pharmaceutics and Medicinal Chemistry; Biotechnology, Toxicology; analytical scientists in government, pharmaceutical industry and biotechnology industry and regulatory agencies.

Table of Contents

    1. GENERAL PRINCIPLES OF ANALYTICAL INSTRUMENTATION

    1.1 Introduction

    1.2 Experimental Studies

    1.3 Experimental Errors

    1.4 Statistical Parameters for Validation of an Experiment

     

    2. SOLUTIONS AND BUFFERS

    2.1 Introduction

    2.2 Units of Concentration

    2.3 The Concept of pH

    2.4 Acids and Bases

    2.5 Henderson–Hasselbalch Equation

    2.6 Determination of pKa

    2.7 Buffers

     

    3. MICROSCOPY

    3.1 Historical Background

    3.2 Nature of Light

    3.3 Compound Microscope

    3.4 Image Formation in a Light Microscope

    3.5 Phase Contrast Microscopy

    3.6 Fluorescence Microscopy

    3.7 Electron Microscopy

     

    4. CELL DISRUPTION

    4.1 Introduction

    4.2 Barriers for Cell Disruption

    4.3 Methods of Cell Disruption—an Overview

    4.4 Mechanical Methods of Cell Disruption

    4.5 Non-Mechanical Methods of Cell Disruption

    4.6 Combinations of Cell Disruption Methods

    4.7 Selection of Cell Disruption Methods

    4.8 Analysis of Cell Disruption

     

    5. CENTRIFUGATION

    5.1 Introduction

    5.2 Principles of Centrifugation

    5.3 Centrifuge Machines

    5.4 Centrifugal Separations

    5.5 Analytical Ultracentrifugation

    5.6 Care and Safety of Centrifuges

     

    6. CHROMATOGRAPHIC TECHNIQUES

    6.1 Introduction

    6.2 Types of Chromatographic Techniques

    6.3 Planar Chromatography

    6.4 Column Chromatography

    6.5 Protein Purification Strategies

     

    7. ELECTROPHORESIS

    7.1 Introduction

    7.2 Principles of Electrophoresis

    7.3 Free Solution Electrophoresis

    7.4 Paper Electrophoresis

    7.5 Gel Electrophoresis

    7.6 Gel Electrophoresis of Proteins

    7.7 Gel Electrophoresis of Nucleic Acids

    7.8 Capillary Electrophoresis

     

    8. SPECTROSCOPY I

    8.1 Electromagnetic Radiations

    8.2 Ultraviolet and Visible Light Spectroscopy

    8.3 Fluorescence Spectroscopy

    8.4 Atomic Absorption Spectrometry

    8.5 X-ray Spectroscopy

    8.6 Circular Dichroism and Optical Rotatory Dispersion

     

    9. SPECTROSCOPY II

    9.1 Introduction

    9.2 Infrared Spectroscopy

    9.3 Nuclear Magnetic Resonance Spectroscopy

    9.4 Electron Spin Resonance Spectroscopy

    9.5 Mass Spectroscopy

     

    10. RADIOISOTOPE TECHNIQUES

    10.1 Introduction and Applications

    10.2 Structure of Atom and Radioactivity

    10.3 Types of Radioactive Decay

    10.4 Interaction of Radioactivity with Matter

    10.5 Kinetics of Radioactive Decay

    10.6 Units of Radioactivity

    10.7 Detection and Measurement of Radioactivity

    10.8 Safety Issues and Radio-waste Management

     

    11. IMMUNOCHEMICAL TECHNIQUES

    11.1 Introduction

    11.2 Production and Purification of Antibodies

    11.3 Immunoassay Techniques

    11.4 Advances in Immunochemical Techniques

     

    12. MOLECULAR BIOLOGY TECHNIQUES

    12.1 Introduction

    12.2 Isolation of Nucleic Acids

    12.3 Qualitative and Quantitative Evaluation of Nucleic Acids

    12.4 Nucleic Acid Hybridization

    12.5 Restriction Digestion

    12.6 Nucleic Acid Sequencing

    12.7 DNA Amplification by Polymerase Chain Reaction 

Keywords

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